Ok, let me make sure I understand correctly. You want to limit it so that only certain groups in AD can login to Serv-U? If so, the easiest way to do this is to only define home directories for the groups that you want to access Serv-U. Be sure not to have a home directory defined for the Default Windows Group as every AD user is a member of that group. This way only people belonging to the groups with home directories will be able to login. Other users will be denied access as they won't have a home directory.
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